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Labs testing for Little Cherry Virus and X-disease Phytoplasma

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University Labs

The Oregon State University Plant Clinic can test for both Little cherry virus 1 and 2, as well as X disease (Western X). For information, please visit their web site at https://bpp.oregonstate.edu/plant-clinic. A list of services and fees are available at the “Submit a sample” pull down menu.

WSU Plant Pest Diagnostic Clinic can run X-disease phytoplasma and Little cherry virus 2 samples with a limited capacity. https://plantpath.wsu.edu/diagnostics/ Choose Virus Assay Request. Please email the lab for projected turn-around times before sending samples rachel.bomberger@wsu.edu so as to not overwhelm lab capacity. Proficiency test panel PASS X-disease phytoplasma PASS LChV2 Aug 8, 2020.

Commercial labs which have passed proficiency testing

In order to help the industry manage X-disease and Little cherry disease WSU is providing support for commercial labs including updated protocols, positive controls, training where possible and proficiency testing.

The following labs:

  1. Have a signed MOA with WSU agreeing to use WSU X-disease phytoplasma and Little cherry virus 2 testing protocols last updated June 2020.
  2. Have been provided with positive controls from WSU.
  3. Have passed a proficiency test (Ring test) where WSU provided samples (12) which were tested using the same protocols by WSU and said lab. To pass the panel positive and negative results match and Ct values must fall within 3 cycles. Extremely low concentration samples (>37 Ct) where the fact that samples were from the same tree and branch but not the exact same sample are excluded from proficiency panel analysis. Individual labs have two opportunities to pass the panel. In 2020 two panels have been provided the first with random samples from grower orchards. The second panel included known negative and known positive samples with homogenized tissue.  Proficiency testing protocols download here.

AgNema www.agnema.com 1320 E Spokane St, Suite A Pasco, Washington 99301 509-255-3744 info@agnema.com Choose forms > Little Cherry Disease sample submission form Initial panel passed. Final panel PASS X-disease phytoplasma PASS LChV2 Aug 8, 2020. 

 

Proficiency testing on-going.

Additional commercial labs

Eurofins Cascade Analytical. 1008 W. Ahtanum Rd. Union Gap, WA 98903. (509) 452-7707 For courier service and additional drop locations visit http://www.cascadeanalytical.com Proficiency test panel 1 completed. Proficiency test panel 2 provided July 23. WSU provided positive controls and updated protocols. Signed MOA in place.

Washington State University